Description
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-TGF- beta 2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-TGF-beta2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGF-beta2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGF-beta2 can be calculated.
Background: Transforming growth factor-beta 2 (TGF-beta2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is known to suppress the effects of interleukin dependent T-cell tumors. It is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma. In 2004, Gottanka found that TGF-beta2 reduced outflow facility when perfused into cultured Mouse anterior segments. Furthermore, TGF-beta2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility